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Query: [V] COG1002 Type II restriction enzyme, methylase subunits, from VFDB

Results of FFAS03 search in VFDB
Master-slave alignment(slide right to see more) does not show gaps in the query sequence, use ali links to display alignment between query and templates.
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# Score Template Links and tools%idFirst MDKSAIIEFSNKVRNKLNEEVKSQAAYYGIFPKEIHSVEEHPDSVVINGSVFNSKIKNQRSQLVKQIKEKGYEQVMEEVTYTWFNRLVALKFMEMNGCLQDQIKVFTSTDPNKPEPDLLTNALKLDFLNLNRDLVLDLKAENKDEELYKYLILKLCNYLNKIMPFLFEKIEDYTELLFPDKLLHTGSVLHDLNSIIPDEDWQEVEIIGWIYQDYIAPKKDKVFADLKKNIKISKENIPAVTQLFTPHWIVRYLVENSLGRLWMLNRPESSIVDLMEYYVKPEQQETDFLKINSPEELKICDPACGSGHMLVYAFDLLYAIYEEEGYTPSEIPEIILTHNLFGIEIDKRAGELAGFALTMKARGKDRHFFKKQVQPNICMLENITFEQSELDAYIDAVGSELFTNAHRATLLQFAEADNFGSLIRPAAMEVSGLLSLLESKNLSGNLSHLSTHQKVLQALKQADYLSPKYHVVIANPPYMGNKGMNNRLKTFLQNNYADVKSDLFSAFIVRNTELAFPKGQLGFMSPFVWMFISSYEKLRDFLINEKTITSLIQLEYSGFDGATVPICTFTSENAYHPEFKGGYVRLSDFRGAENQSPKTLEAIKNPKCGWFYRASATDFKKIPGNPIAYWVSNKVRDLFGNITLGDFFDTEGQNKTTDNDKYLKYHWELREKTIGVDKKWVLCAKGGSFRKWQGNIEYVIDWSPEARAFYRKDHSARIVEEEFWYREGITWTGITSSLSGFRYLEYGCTYETAGPTIFVKNANKYVILGYLNTPIVAKFINIMNPTLNTKIKDVQALPIIQSILFDNQVESVSLRLVNLSKSDWDSYETSWDFTTLPFLQSEFRQPTLSETYTKLRTHWKEMTLEMQRLEEENNRIFIEAYGLQDEITPEVPLSEITLTCNPYYRYGNNKTEEELETLLMTDTVKELVSYAVGCMFGRYSPEKEGLILANQGEKLGDFKEKVPRATFLPDEDNIIPILDDEYYTDDIVGRFKEFLKHTFGAETLSENLNFIAGAISKKNESPGKVIRDYFLKDFYKDHVKMYKKRPIYWLFTSSEKGKAFNALVYMHRYDKTTLAKMRIDYLLDFESKLDAQRSLLKKEITENSKNSGKAESELAKLNKKINELVKYDELLKNKADQMIEIDLDDGVKVNYEKFKGLVGKILast
1 -298.000[V] COG1002 Type II restriction enzyme, methylase subunits  ali  100  1MDKSAIIEFSNKVRNKLNEEVKSQAAYYGIFPKEIHSVEEHPDSVVINGSVFNSKIKNQRSQLVKQIKEKGYEQVMEEVTYTWFNRLVALKFMEMNGCLQDQIKVFTSTDPNKPEPDLLTNALKLDFLNLNRDLVLDLKAENKDEELYKYLILKLCNYLNKIMPFLFEKIEDYTELLFPDKLLHTGSVLHDLNSIIPDEDWQEVEIIGWIYQDYIAPKKDKVFADLKKNIKISKENIPAVTQLFTPHWIVRYLVENSLGRLWMLNRPESSIVDLMEYYVKPEQQETDFLKINSPEELKICDPACGSGHMLVYAFDLLYAIYEEEGYTPSEIPEIILTHNLFGIEIDKRAGELAGFALTMKARGKDRHFFKKQVQPNICMLENITFEQSELDAYIDAVGSELFTNAHRATLLQFAEADNFGSLIRPAAMEVSGLLSLLESKNLSGNLSHLSTHQKVLQALKQADYLSPKYHVVIANPPYMGNKGMNNRLKTFLQNNYADVKSDLFSAFIVRNTELAFPKGQLGFMSPFVWMFISSYEKLRDFLINEKTITSLIQLEYSGFDGATVPICTFTSENAYHPEFKGGYVRLSDFRGAENQSPKTLEAIKNPKCGWFYRASATDFKKIPGNPIAYWVSNKVRDLFGNITLGDFFDTEGQNKTTDNDKYLKYHWELREKTIGVDKKWVLCAKGGSFRKWQGNIEYVIDWSPEARAFYRKDHSARIVEEEFWYREGITWTGITSSLSGFRYLEYGCTYETAGPTIFVKNANKYVILGYLNTPIVAKFINIMNPTLNTKIKDVQALPIIQSILFDNQVESVSLRLVNLSKSDWDSYETSWDFTTLPFLQSEFRQPTLSETYTKLRTHWKEMTLEMQRLEEENNRIFIEAYGLQDEITPEVPLSEITLTCNPYYRYGNNKTEEELETLLMTDTVKELVSYAVGCMFGRYSPEKEGLILANQGEKLGDFKEKVPRATFLPDEDNIIPILDDEYYTDDIVGRFKEFLKHTFGAETLSENLNFIAGAISKKNESPGKVIRDYFLKDFYKDHVKMYKKRPIYWLFTSSEKGKAFNALVYMHRYDKTTLAKMRIDYLLDFESKLDAQRSLLKKEITENSKNSGKAESELAKLNKKINELVKYDELLKNKADQMIEIDLDDGVKVNYEKFKGLVGKI 1161
2 -33.900[V] COG0286 Type I restriction-modification system methyltransferase subunit  ali follow..  14  2..................................................SISTTIKTIQDIMRKDAGVDGDAQRINQLVW-----MIFLKVFDAR--------EEEYELLEDNYKSPIPEGLRWRNWAADSEGITGDGLLDFVDNALFKTLKELRTTATDARGQMIGKVEDAYNYMKNGTLIRQ---VINKLNEVDFNKKDQKKQFSEIYEKILKD-----------------QSAGNAGEYYTPRAVTKFIVD----------------------RIKPQLGE------------IVFDPACGTGGFLTAAIDYIRQ------HFQSADVPEILQRTIRGTEKKPLPFNLCVTNLILNGIDVP-------------------------------------------------SAEHDNTLARPLRDYSP----------------------------------HERVDVIITNPPF--GGMEEDGIEDNFPATFRTRETAD--LFLVLIAHLLKEGGRGAIVLPDGTLFGEGVKRIKEKLLQDCNLHTIVRLPNGVFNPYTIKTNLLFFTKGEPTETIWYYEHPYPAGYKSYSKTKPIRFEEFAPEQEWWDNREEN............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... 413
3 -22.800[R] COG4889 Predicted helicase  ali follow..  11  844..........................................................................................................................................................................................................KQAVIKDLYERFFK--------------KAFKKQSEALGIVYTPVEIVDFILR-AADDVSKKHFGRGLSDK----------------------DVHVLDPFTGTGTFMVRLLQSGLI--------KPEDLARKYANELHATEIMLLAYYVAAVNIETTYFGLEGERALRN-------------------GEDAPVYEPFDGIVLGDTFQMYEDDDKLDLDVFTANNDRMERQR------------------------------LTPVQVIVGNPPYSVGQSIEDSYAKYSTATNKNSLYDSYLRAFRWATDRIHTQGVVAFVSNNGWVDGNTADGVRLSLAQDFSEIYVFNLGGNVFNVRVGTQIIVAVKNPQLSGCRILYKDIGDNLSADAKLNEIAVA----------TIEGAEWQTISPNEYGDWISQ-------------------------------------------------------------------------------------RSVDFDTWPVLGDKKNKSALKVFQTFSAGLKTGRDAWCYGPTSAQVKTNITRLLETYEQAQQRFNSWVVDNGVTSPKEADVNQFLKQNPDLADSKKISWDSNLKMSLSRGDTFSFDPSSIQMSLYRPFFPQQTYFHVSLNQRRYQLPSMFPTPEHDNQGFYIVNPGSAKPFSTLATNL--------------------------PDLAMWGSNAGQFFTRWTWEPIETREGELDFGNGLFSTTPKKGVEGEILDGY---------VDNITDEILKLYQSSLGEDTKDDIFYFVYAQLHDPA----------YREAYAADLKKMLPHIET------PTDRARFKELMDLHINYEDVEPWDVEVKVKEKADPTDRETWRVTKMKWAKVRDPETKKLVEDHTTLIYNS----SITISGIPEEAENYQLG-SRSA............ 1587
4 -20.100[J] KOG3420 Predicted RNA methylase  ali follow..  11  6.............................................................................................................................................................................................................................................................TLWMLNELEGFEKPKIKLEQYATSSELAVSMMEMIDETIGFEGKKLIDIGCGCGMLMTTAATMY-------------------LETVLGVDIDDEALKICSRNLETAEVQDRCELLQADI------------------------------------------------------------------------------------LDPESDLPRGTFDVAVINPPFGTKN-----------------NAGIDMQFVQIGLQMVRPGGSVFSLHKSSTRNAKKWDGVGAECCAEMRWQLPATYKFHKQKAVDIAVDLIHFKKLDS......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... 214
5 -17.400[L] COG0827 Adenine-specific DNA methylase  ali follow..  20  421...................................................................................................................................................................................QANIAAIRLLHRLETENRFANSEERNILGGLADAFDENKLAFQKEYLELKELLTPEEYQAATTYYTEPALIDRMYQQGLN----------------------------------VEPEKILEPSMGIGHFF--------------------LPKAYQTASLTGVEIDKLSGRIAK-KLYPKA---------------------------------------------AIQLKGFEEMN----------------------------------------------SQSNTFDIAIGNIPFNDFN--------VTDKSKRYPHKLIHDYFFEKSLDLVKPGGYILFVTSKGTM--KKDSRARHYLAQRADLISAVRLPNTAFSGTKATTDILLLQKLEKERF...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... 683
6 -14.400[J] KOG3191 Predicted N6-DNA-methyltransferase  ali follow..  12  1.............................................................................................................................................................................................................MSPQKVSQKCKKPMSMPTPQYRLQAIRAHD-------VYEPAEDTFLLIDAIEKDI---------------------------KEIRSRDPKLVLEIGCGSGVVSTFVNQAL-------------------NVTSVATDLNPHALDVTL---------------------------------------------------------------------------------------ETAKLNDIKIDVVRTDLFAGLENLLGKVDVLLFNPPYVPTDEEPKSNIELTYAGGRTGR-STLDRLLPRVPELLSPRGVFYLVALHS......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... 184
7 -14.000[J] COG2263 Predicted RNA methylase  ali follow..  15  2..........................................................................................................................................................................................................................KQRKLEMLLEEVEGFSSPELELEQYQTPSPLAAEILHFAY--------------------------------MQGDLDESVQDLGCGTGILAIGA-------------------KLLGARKVVGYDTDPKALEVAR-----------------------------------------------------------KNAERLGVEVEFVCSDIKKV--------------------------------SGHVKTTLMNPPFGARVKGRD--RPFLSSALRTSEV-IYSIHNRGSLAFIQK.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... 155
8 -13.700[L] COG1041 Predicted DNA modification methylase  ali follow..  14  37.....................................................................................................................................................................LVSGSVKHIGVFERLGLAHEYGLLLYEGDDIKDKGLEWRNIINGTFAVRRERMRNCMHEVNDLDKKIGGIIHSQGLRLSRPDTVVRVYCGNKLGIRIREFKGKEFEDRKADRRPFSRPIALPPRIARAMVNLEVLDPFMGTGGMLIEA--------------------GLIGLKVYGLDIREDMVEGAKINL----------------------------------EYYGIRDYVVKVGDATKIEEVFP---------------------------------------------------GKTFEAVATDPPYGNSTTLPMDRN------------ELYKRSLESIHSVLE--GRLAIAFPADFDARDVAESIGFKVLGRFYQRVHSSLSRYFY.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... 325
9 -12.800[L] KOG2671 Putative RNA methylase  ali follow..  9  55......................................................................................................................................................................LSSEEIARNIAKRSILVKGMYELWGEGTCYEELKDSIQSYPDSRKLPFLTSDSTFRISVETFGKALTFDEQKDR-NLKNPDHNFFLMEMIEFGREVGFADRKLLPTFQLKSRTYLGPTAMDAEMAFLMANQAVYDPFVGTGSILVSA--------------------ARFGAMTMGADIDIRVVRDGR----------------------------------------------------GPDCNVWSNFKQYGLPMPVALLRMDNNVPPWRSG------------------------LKEIFDAIICDPPYGVRAGGRKSGGRKILRGTVDPYTECVHDLLHLAARMLVMKGRLVFFFP----VLRDETGSEVKFPEHPCFKLVAVSEQIL--SSRYSRVLLTMVKVEPYSEE..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... 420
10 -12.700[R] COG4123 Predicted O-methyltransferase  ali follow..  13  50.........................................................................................................................................................................................................................................................................................................LIVDFCAGNG-----AVGLFASTRTQA--------------QILSVEIQERLADMAERSVRLNGLEEQMQVICDDLK------------------------------------------------------------------------------------NMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSAQSILKSNGRLAMVHRPDRL-LDILDTLKRHNLAPKRLQFV-------YPKREKEANMLLIEAIKDGSTSGFKVL................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ 226
11 -12.200[R] KOG2904 Predicted methyltransferase  ali follow..  10  108.....................................................................................................................................................................................................EDSIVDDPLV-ADNGEKNVKLRASLEELYDLWRQRIEKRRPVEEGVLIPRPETELIVDMVEELV---------------------------TRDEWFKKDIWADLGTGSGAIAIGIAKVLGS-----------------RGRVIATDLSPVAIAVAG-----------------------------------------------------------HNVQRYSLEGMIEVREGSWFEPLKDL--------------------------EGKLVGLVSNPPYIPSDGLQAEVGRHEPKLALDGGIDSLFHLCHGASRMLQPGGFFVFETNGE----KQSKMIVDYMMTSDLKDCFSDL--KIVSDFAGINRFVTGFR............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ 376
12 -12.100[J] COG2890 Methylase of polypeptide chain release factors  ali follow..  11  42...............................................................................................................................................................................................TDNAWDEAVQLILPTLYLPIDVPPHVLSSRLTSSERLRVVERVIKRINDRT---PAYLTEFFVDSRV---LVPRSPIGELIQNRFEPWLTE------------EPTRIMDLCTGSGCIAI------------------ACANAFPEAEVDAIDISVDALNVAE-----------------------------------------------------------QNIQDHGLEQQVFPIRSDLFRD----------------------------LPQEQYDLIVTNPPYVDQEDMDSLPSEFRHEPELGLAAKLARRILANAPLYLKENGILVC.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. 264
13 -10.300[R] KOG1271 Methyltransferases  ali follow..  18  59......................................................................................................................................................................................................................................................................................................EASRVLDLGCGNGMFLVGLAN--------------------FTGDLTGVDYSPKAVELAQ-----------------------------------------------------------NIAEDNKLSITYKVADLTQPQ-----------------------------NELGQFDVVHDKGTYDAVSLCPDNAKEKRAL------------YLDTVEKLLRTADSLFVITSCNW----TEDELVDSFAEKFVKYYTIPTPTFKFGGKVGNVVTSIVFKRKKE........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ 219
14 -10.300[KL] COG4646 DNA methylase  ali follow..  9  100............................GEPVAVKVRKGRNADGIPEKHVRIIRKLISVRDAVRVVLKAQELDQPWKDLQVRL--RIAWSSFVRDFGPINHTVSITEDPETGKTRESHRRPNLQPFADDPDCWLVASIEDYDLENDTARP-------------GPIFTD-----RVISPPAPPVITSAADALAVVLNERGHVDRDHIAELLHRDPEDVVTEL-----------------GSAIFDGSWQMAYLSGHVREKL-----------------VADAAAGLDPAYERNVSALTAVQPVLGA-AFVKEMMGTDIRIHHMPELASWTVEYLAAGTSEWGTD-RRHAGELLSDALNSRVPQIFDTIRDGDSEKRVLNVVDTEAAKEKLHKIKQAFQRDRLARVYNDRFNNIAPRKFNGDHLKLPGASGAVLYGHQKRGIWRIISAGSTYLAHAVGAGKTMTMAASIMEQLVAKAMQVVPGHCLAQAAREFLALYPTAR---ILVADETNFSKDKRARFLSRAATATW--------------------DAIIITHSAFRFIGVPAAFEQQMIHDELELYETLLLKVEDEDRVSRKRLERLKEGLQERLEALSTRKDDLLTIAEIGVDQIIVDEAQE-FRKLSFATNMSTLKGVDPNGSQRAWDLYVKSRFIETINPGRALVLASGTPITNTLGEMFSVQRL---------GHPALMERGLHEFDAWASTFGDTTTE---LELQPSRKYKPVSRFASFVNVPELIAMFRSFADVVMPADLREYVKVPAISTGRRQIVTSKPTQAFKHYQMVLAERIKAIEEREGPPQPGD---DILLSVITDGRHAAIDLRLVDADNDNEADNKLNNLISN-----AFNIWKATEGSTYLRHDGKSFELTGAAQMIFSDLGTISVEKTRGFSAYRWIRDELI----------RLGVPASQIAFMQDFKKSEAKQRLFGDVRAGLIGSSETMGTGVNAQLRLKALLDVPWLPSQIEQREGRI---VRQGNQHDEVDIFAYATEGSLDATMNNERKARFIAAALSGDTS-QFAMAKAIASGDQRLMQKAG---LEADIARLERLRAAHLDDQHAVRRQIRDAERYVESATRRIAEIGQDIERLVPTSGDAFSMTVMGKVYAERKEAGRALMKE 1183
15 -10.100[R] COG2521 Predicted archaeal methyltransferase  ali follow..  10  81............................................................................................................................................................................................................................................................IAADRFYKLCVFNRGWAPTLMIDGITMHSVLENPLLLTSRKVERARVFECCTGLGYTAIEALKK--------------------ARHVITVEVDPHVLTLASLNPYSRGLWSPA------------------------------------------------------DVILGDCYA------------------------------FVQTVRDSSIDFIIHDPP-----------------RLSHATQRLYSELYREFYRILKRGGGVFHYVSQSGTKYRGLNPYKGVA------ERLRRVG---FAVEKIKEGYGIYARRR.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... 278
16 -9.900[L] COG3392 Adenine-specific DNA methylase  ali follow..  7  1.........................................................................................................................................................................................................................................................MNYI-----GS-------KYKLIPFIKENIHAVAGHDLSGA-------IFCDLFAGTGIVGRTF--------------------KKAVNKVISNDLEYYSFVLNQ-NYIGNIQEIPNKEELINKINSVALKKGFIYSHYSLGGSSRQYFSETNAQKIDAMRLKIEELKLSQNIDNHAFLKRLKKSAQKELILKGAHFDVSLNANEVYQQDSNDLIGKISGDILYLDPPYNARQYGATPKGKTDLPSYQKSSFCSRFQILNAFENLIKKARFKYIFLSYNNEGLMSETEIKNILKKYGAYSLVT--NKRAHKAVHTKECLHVLIK............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ 329
17 -9.590[TDBLU] COG5032 Phosphatidylinositol kinase and protein kinases of the PI-3 kinase family  ali follow..  8  677IGLSALQQIASTRHISVWQLLSPPTVSVAIVQGMGKKPNIASLFAQLMNISEGDFLIRTQAYTLPFLVLTKNKALIVRIAELSMHKILASLLTTDHPNLEESVMLLLSLATSDFEKVDLTSLLRSDPISITVELLQLYQNDVPHEKIENALRKVAMIVSQVVNDEDLSNKELLYDF-FNNHILGILAEFSNILNDLKGKTSINLSLCGGAVKLGLPQILSNLQSAFQNKEPEYSSIAGLSLVILPPLFIFDFISSDTKAKEIFCSLQNEDFYSELQSIIKCLTNENEPVCYLGLQKLELF-----SNEVLDQLLRCLLDCCVKYASTNMQISYLAAK-GELGA-IDPSRAKAQH--------------------------IIKETVVLDNFENGEESLKFILDFMQSQLIPAFLVTTDTKAQGFLAYALQEFLKLGGFKSAVINKKKGLTVVTEHWMSLPDLSFLTSKYHL-IRYPIYKENVTIHTWMQLFSLKLMEYAHSQNAEKIFGICSKVVKDQEV----------------NIPCFLLPFLVLNVILTESELEVNKVIEEFQLVINQPGPDGLNSVVFFKIVDYLNKWLRMRKKRNWDRRSAIARKENRYMSVEDATSRESSISKVESFLSRF------PSKTLGIVSLNCGFHARALFYWEQHIRNATA---------PYAALESDYRVLQEIYAGIDDPDEIEAVSLNFHDYSFD---------QQLLLHENSGTWDSALSCYEII---IQKDPENKKAKIGLLNSESLVLSLDSFIINDNHEYSKMLNLGIEAS---------RSLSIDSLKKCLSKSNLESFEAKLGSIFYQYLRKDSFAELTERLQPLYVDAATAIANTGAHS-DILSKLHAINDFSRIAETDGIVSDNLDIVLRRRLSQVAPYGKFKHQILSTHLVGIARISRKNGQFQRAFNAILKAMDLDKPLATIEHAQWWWHQGQHRKAISELNFETLGNPLKGKVF-LKLTKWLGKAGQLGLKDLETYYHKAVEIYSECENTHY-------YLGHHRVLMYEEEQKLPVNEQSGRSLYYGTNHIYESMPKLLTLWLD-----FGAEELRLSKDDGEKYFREHIISSRKKSLELMNSNVCRLSMKIPQYFFLVALSQMISRVCHPNNKVYKILEHI 1921
18 -9.410[R] KOG2899 Predicted methyltransferase  ali follow..  10  49.......................................................................................................................................................................................................................TSRPPAQSPKKRLHLNGKPMQNKDLNFKY-----------GNYKHYYGKRILNKDFHDIRLDVLGT--------QPDLFRNKQLLDIGCNSGHLSI------------------QIARKFEVKSLVGLDIDRGLINDAQKTVSHLKRHATPGQGIPHIQFVHGNYVLEDDVLLE--------------------------------------------------------------------------IERPQFDVILCL-----------SVTKWIHLNFCDSGLK----AFRRMYLQLRPGGKL-ILEPQSFDGYKRRKKLSVLLSPEVGFAEMKLMGIPEHCKVGFKRPIQIFTKS........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... 323
FFAS is supported by the NIH grant R01-GM087218-01
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Selected papers from Godzik Lab
Ying Zhang, Ines Thiele, Dana Weekes, Zhanwen Li, Lukasz Jaroszewski, Krzysztof Ginalski, Ashley Deacon, John Wooley, Scott Lesley, Ian Wilson, Bernhard Palsson, Andrei Osterman, Adam Godzik. Three-Dimensional Structural View of the Central Metabolic Network of Thermotoga maritima. Science. 2009 Sep 18;325(5947):1544-9.

Mayya Sedova, Mallika Iyer, Zhanwen Li, Lukasz Jaroszewski, Kai W Post, Thomas Hrabe, Eduard Porta-Pardo, Adam Godzik Cancer3D 2.0:: interactive analysis of 3D patterns of cancer mutations in cancer subsets. Nucleic Acids Research, gky1098 2018; Published on November 8 2018.

Li W, Godzik A. cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics. 2006 May 26;